Coding

Part:BBa_K4812014:Design

Designed by: Rashik Chand   Group: iGEM23_NYU-Abu-Dhabi   (2023-10-09)


Scl2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 454
    Illegal PstI site found at 498
    Illegal PstI site found at 561
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 454
    Illegal PstI site found at 498
    Illegal PstI site found at 561
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 454
    Illegal BamHI site found at 891
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 454
    Illegal PstI site found at 498
    Illegal PstI site found at 561
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 454
    Illegal PstI site found at 498
    Illegal PstI site found at 561
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 551


Design Notes

Manufacturability of part. Repeated sequence makes it difficult to synthesize.


Source

Group A Streptococcus (GAS)

References